Han Chunyu NgAgo technology in deep quagmire of repeatability, Korea scholar published an article said NgAgo in vitro RNA (ribonucleic acid), rather than as claimed by the Han spring paper cutting in the mammalian cell DNA (deoxyribonucleic acid).
In November 2016, on the nature of biotechnology, Korea Basic Science Institute’s Center for gene editing Jin Zhenzhu (Jin-Soo Kim) team together with the other two teams, communications issued a commentary article, project group of Hebei University of science and technology Han Chunyu NgAgo technology failed to detect DNA-led genome editing, questioned Han Chunyu NgAgo repeatability of experiments.
Almost 2 months later, on January 20, in the life science preprint on the site BioRxiv, Jin Zhenzhu published another article about NgAgo technical papers. BioRxiv by the nonprofit United States Cold Spring Harbor Laboratory was founded to accelerate research dissemination, in scientific papers began before the peer review, enable them to make paper feedback opportunities.
In the paper, Jin Zhenzhu not deny review communications the conclusion that two months ago, cannot repeat experiments were mentioned again in the article. But he found on another – in vitro NgAgo, NgAgo as guided by a DNA endonuclease cuts RNA.
This means NgAgo might serve as “RNA interference” a tool to play a role. Silence is a form of RNA interference genes, which did not express specific genes, which can be used in disease treatment through gene “silence” to achieve a therapeutic effect.
According to the report on January 20 in the journal nature, revealed Jin Zhenzhu has recently applied for a patent on the gene silencing using NgAgo.
Worth noting is that Korean spring research group in May 2016, published in the journal Nature biotechnology on NgAgo technical papers, reported in mammalian cell systems, by DNA-mediated, NgAgo can efficiently cut editing DNA. And report Jin Zhenzhu NgAgo characteristic not consistent-RNA-a cutting, a cutting of DNA, correspond to different concepts.
Earlier, in November 2016, Nantong University and Fudan University team, published in the cell (Cell Research) and Jin Zhenzhu latest conclusions of a study on ECHO.
Article published in the journal cell research papers show, the research team used in the cells of the Zebrafish NgAgo technology, eye development of the Zebrafish defects occurred, suspected gene editing. But found after sequencing, genome-wide level or DNA level, without any editing. Further, they found that only occurs on the mRNA expression of the change. This means the reasons causing development defect gene is low, rather than gene editing.
Turbulent news has also introduced gene and gene edits differences. In simple terms, low gene is RNA interference effect on gene expression, gene editing is to interfere with the DNA, completely influencing gene expression. Differences in results can be understood as both a hammer and a pair of scissors.
From the life of running processes to start. DNA is responsible for guiding development of biological life and functioning of “Commander”, but the exact biological function of proteins to perform. Therefore, DNA needs to be transcribed into RNA, through the translation of RNA into proteins. This process is known as gene expression, in which RNA acted as an intermediary role. Li keqiang interpretation of international experts
Relationship between DNA and RNA. Micro letter public, “Mr”
If through artificial means, this intermediary RNA interference, gene expression, had completed only a handful of gene expression, protein does not work, we will refer to this phenomenon as “deficient”. If DNA is the “Commander” changes, changes the sequence of the gene, which is a higher level of intervention will totally alter gene expression, we call it “gene editing”.
In addition, the surging News learned that NgAgo protease is Ago a family member of the Ago conclusion of RNA degradation, mediation is not new, but Ago through RNA-guided, some through DNA-guided. Jin Zhenzhu’s latest research reveals how NgAgo intervention mechanism for RNA.
It is worth mentioning that, more and more signs point to NgAgo technology may have potential in gene silencing, rather than gene editing.
In a report in the January 20 issue of nature, said Han Chunyu, Novozymes evidence NgAgo gene regulation, and said Novozymes recognized this. But the journal nature noted that gene through gene editing can also be achieved by gene silencing. Han Chunyu nature that, before releasing more information regarding patents, he have to speak to a lawyer.
January 19, two dynamic allows Han Chunyu NgAgo repeatability controversy has made new progress.
At noon that day, nature biotechnology that won and NgAgo new data related to system reproducibility requires investigation. Submitted by Han Chunyu surging news that this is their team, and deny is the original data.
Then, that afternoon, Hebei University of science and technology official website posted the “Hebei University of science and technology of gene editing Technology Research Centre signed a cooperation agreement with Novozymes company” article. The next day, admitted in a statement reached at NgAgo Novozymes company cooperation. Hebei University of science and technology and Novozymes company avoided mention in the repetitive questions, only represent NgAgo “fungal expression system” and the “production of enzymes of microbial expression systems” there is potential.
Australia University Centre yuehan·Ke Ting geneticist jiaaitang·buer Coke (Gaetan Burgio) is the first publicly questioned NgAgo reproducibility experiment one of the biologists, he conducted experiments in the fertilized eggs in mice, but have not seen any signs of gene editing.
Burgio told the journal nature, said in an interview, Novozymes has an for gene silencing by RNA interference technology, Burgio speculated that Novozymes Hebei University of science and technology and gene-editing technologies Research Centre may be found a more effective way to silence genes.
For Novozymes and gene editing technology research center of Hebei University of science and technology cooperation, Wei Wensheng, a professor in the school of life sciences, Peking University nature says: “this doesn’t change anything. Han Chunyu (in the paper) said NgAgo gene editing in mammalian cell systems efficiently. Then prove it! ”